Definitely not, enshitification is real. I’ve gone from someone excited about new products to I don’t trust anything that I didn’t build. We are currently discussing moving our group chat off discord, but I’m biochemist and my circle are similar professions and I’m still learning how to properly host things.

In the before times we used hamachi, but it was hard. Eventually, Skype was born and it was good and easy. It worked like a phone and many StarCraft 2 team games were won. But Skype was corrupted by the many Nigerian princes and hot Ladies who only needed a few dollars to turn your life around. This corruption was then baked so deeply into windows it took registry edits to exorcise it. But all was good was we now had discord which took the ideas of hamachi and Skype and delived a better experience than both.

You’d be surprised how much money gets wasted of stupid projects and acquisitions in biotech because some suit think they understand science better than their R&D team. For analogy sake think about all the stupid shit Microsoft bought and killed or all the chat apps Google created and killed, this going to be Regeneron’s Skype.

If you really want to get your parents sequenced for your own personal use without it going into a database it’ll cost you about $500 per sample (cheaper if you know someone who can extract the DNA for you). You’ll get a set of fastq files for the reads that will cover almost their entire genome that you can then use with public databases or just store for future use. Another option is to sign up for a university study but you’ll have to be comfortable with their data use.

I honestly don’t know what they will do with snp data. These investors and VCs have been running scare peace articles for the last two years to drive the company into bankruptcy so that it could be sold and the data harvested. But I honestly think people are really overestimating the value of a dataset showing how different people are from a standard template. It’s good for ancestry and correlations but people forget they didn’t fully sequence samples. I fully expect the news cycle to change once they figure this out as they try to get people to resubmit DNA for nextgen sequencing, so they can try to salvage their investment.

This was almost my gaming PC specs in 2020. Rx580 and 16gb more ram. It’s now my server running jellyfin and immich for my family.

Slowly being turned towards the sea

I’m 11hrs in so far, and I’m hooked. The world building, gameplay and characters are amazing. I also really loved the music, especially inside the manor.

Thanks the heavens, I’ve been using the browser on the Xbox for jellyfin.

Need more information to properly diagnose. Is this a new issue? Are you using auto build leveling for every print? Have you tried cleaning everything and rerunning the system calibration? A lot of different things can cause similar issues and it’s hard to diagnose from images alone.

Paying people to review articles is just going to make things worse. The real problem is open access. In the current system the author of the manuscript has to pay to publish it, and the publisher turns around and asks readers to pay to access it. Its a scam. If research was conducted with any public money the knowledge generated should be public. This is why Elsevier needs to go. If you saw how much money institutions have to pump into these useless publishers to get access to knowledge funded by the public for the public there would be more outrage.

I just bought myself a Bambu P1S, after building and modifying ender3s since 2019. I love tinkering and customizing things but it’s nice to have a printer that just works. Its so reliable that my wife can now print her own stuff using the app instead of giving me a list. The only thing I really have to do is change the filaments loaded in the AMS for her. If you have no experience printing I would just get a A1 mini and use it until you run into the limitations.

If you live near a college campus, check out their recycling center or campus surplus. Lots of IT departments just toss UPS when they upgrade equipment regardless of battery status. I like to grab APC ones because the battery replacements are easy to find and there’s usually a YouTube video for the exact model with someone refurbing it.

They really should start selling it and marketing it as never frozen fresh. It’ll be even more effective that way once its legal nationwide.

Me: Everytime I got time on the confocal

Halo

I’ve been buying used 12tb HGST from Amazon for $80.

Lol yeh but it’s partially funded by money saved from canceled subscriptions.

I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.

Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

• the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.